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LY3298176 activated both GIP and GLP-1 receptor signaling in vitro and showed glucose-dependent insulin secretion and improved glucose tolerance by acting on both GIP and GLP-1 receptors in mice

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A 0.5 ml portion of each assay preparation was added to a well in a 24-well plate, and they were incubated at 37 C in 5% CO 2 for 1 h