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The velocity of such a ping-pong reaction with infinite true k cat and K m values can be described by the equation [ E ]/ V = 1 /[GSSCys]+ 2 /[GSH], wherein the Dalziel coefficients 1 and 2 are the slopes of secondary plots for k cat app (GSH) and k cat app (GSSCys) , respectively 39 (Supplementary Table 1)
Storage Recommendations: Store in a tightly closed container under inert atmosphere (nitrogen or argon) at 28C, protected from moisture and light to maintain stability
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They are typically transient and do not require stopping the protocol
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