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HRP-labeled rabbit anti-rat IgG (ab6734) was purchased from Abcam (Cambridge, MA, USA)
Brain sections were thawed for 30 min at 23C, and then pre-incubated for 30 min in 50 mM Tris HCl buffer pH 7.4 at 23C
doi: 10.2337/dc12-2454 72 RosenstockJHanefeldMShamannaPMinKWBokaGMiossecPet al
Other weight-loss drugs approved for long-term use, like naltrexone/bupropion (Contrave) and phentermine/topiramate (Qsymia), suppress your appetite in different ways, like targeting nerves in the brain
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