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if you're still in dose-escalation phases, waiting for full tolerance before judging efficacy prevents premature conclusions about medication failure

Ten microliters of 0.1 mg mL 1 ShHTL7 protein in a reaction buffer containing 20 mM HEPES, pH 7.0, 150 mM NaCl and 80 L of reaction buffer were mixed in a 96-well black plate (Grenier)

Therefore, giving diets containing plant meals to fish will often induce massive changes in the intestinal microbiome, with a selective advantage to those microorganisms that are tolerant to or even able to utilize these antinutritional factors

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