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Biotin-labelled proteins were eluted by boiling in 2 Laemmli sample buffer for 5 min prior to IB analysis
Lee B-H, Hu Y-F, Das SP, Chu Y-T, Hsu W-H, Nan F-H (2024) An in vitro system mimics the intestinal microbiota of striped beakfish ( Oplegnathus fasciatus ) and inhibits vibrio alginolyticus by Limosilactobacillus reuteri -derived extracellular vesicles
But individual responses vary, and monitoring is appropriate
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