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In the scRNA-seq analysis, cells mapping to clusters indicative of gonadal contamination were excluded before final clustering and annotation

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For injection site reactions: Apply ice before injection to reduce discomfort Ensure proper technique Rotate injection sites consistently Use smaller injection volumes if reactions persist Consider different injection locations For systemic effects: Reduce the dose if effects are bothersome Adjust timing of administration Ensure adequate hydration Monitor for resolution (often within days) Discontinue if effects persist or worsen For digestive changes: Usually beneficial and not requiring intervention Adjust timing relative to meals if needed Reduce the dose if effects are uncomfortable Monitor for resolution General principles: Document all adverse effects Assess severity and relationship to the peptides Consider dose reduction before discontinuation Provide supportive care as needed Discontinue if serious effects occur (extremely rare) Long-Term Safety Considerations Most research covers short to medium-term use (412 weeks)